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Morphological and ecological differentiation and distribution of taxa in the Galium mollugo group
Talpová, Julie ; Kaplan, Zdeněk (advisor) ; Štech, Milan (referee)
My thesis deals with three taxa of the polyploid complex Galium mollugo that occur in the Czech Republic. These are diploid G. mollugo and tetraploid G. album subsp. album and G. album subsp. pycnotrichum. These taxa are very similar to each other, their identification is difficult and exact values of the quantitative characters used for their separation are unknown. To date, there has been no study based on flow cytometry (FCM) and classical morphometrics analysis in this complex. By using them I wanted to bring new results and fill the gaps in scientific research. Primarily, I was investigating whether the individual cytotypes can be distinguished based on different genome size using FCM analyses. Another aim was to determine whether it is possible to define morphological and ecological differences between diploid and tetraploid taxa. The morphometrics included ordination and discrimination methods. I observed whether taxa could be distinguished by the naked eye and determined by measurement which characters are most suitable for identification. FCM analyses proved to be suitable to distinguish the two ploidy levels. I have been able to demonstrate by statistical methods that diploid and tetraploid taxa differ from each other, and I have also refined the characters that are most suitable for...
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Altered morphology of white and grey matter in patients with Alzheimer disease and Schizophrenia on MRI
Lahutsina, Anastasiya ; Zach, Petr (advisor) ; Horáček, Jiří (referee) ; Němcová, Veronika (referee)
Cortical folding of the anterior cingulate cortex (ACC), particularly the cingulate (CS) and the paracingulate (PCS) sulci, represents a neurodevelopmental marker. Deviations in in utero development in schizophrenia can be traced using CS and PCS morphometry. In the present study, we measured the length of CS, PCS, and their segments on T1 MRI scans in 93 patients with first episode schizophrenia and 42 healthy controls. Besides the length, the frequency and the left-right asymmetry of CS/PCS were compared in patients and controls. Distribution of the CS and PCS morphotypes in patients was different from controls. Parcellated sulcal pattern CS3a in the left hemisphere was longer in patients (53.8 ± 25.7 mm vs. 32.7 ± 19.4 mm in controls, p < 0.05), while in CS3c it was reversed-longer in controls (52.5 ± 22.5 mm as opposed to 36.2 ± 12.9 mm, n.s. in patients). Non parcellated PCS in the right hemisphere were longer in patients compared to controls (19.4 ± 10.2 mm vs. 12.1 ± 12.4 mm, p < 0.001). Therefore, concurrent presence of PCS1 and CS1 in the left hemisphere and to some extent in the right hemisphere may be suggestive of a higher probability of schizophrenia. Measurement of an hippocampal area or volume is useful in clinical practice as a supportive aid for diagnosis of Alzheimer's disease....
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Associations of morphometric and metabolic biomarkers with cognitive impairment in Alzheimer's disease and Lewy body dementias
Nedelská, Zuzana ; Hort, Jakub (advisor) ; Nevrlý, Martin (referee) ; Dušek, Petr (referee)
Associations of morphometric and metabolic biomarkers with cognitive impairment in Alzheimer's disease and Lewy body dementias Abstract Dementia has become one of the major health care and socio-economic challenges. Alzheimer's disease (AD) is the most common dementia whereas dementia with Lewy bodies (DLB) is the second most common neurodegenerative after AD. However, both dementias exist in a quite heterogeneous contiua that can overlap with each other. Approaches that allow for the identification of individuals at risk of developing AD in preclinical or prodromal stages are of major interest to apply the symptomatic and newly introduced biological therapies and non- pharmacological interventions that are more effective early on. Similar efforts are undertaken in the DLB field although no causal treatment for DLB is available yet. A prerequisite for an efficacious and targeted intervention is a selection of individuals who would benefit the most from this intervention. This process includes the timely and accurate diagnosis, differential diagnosis, prognostication, and management of treatable comorbidities. This dissertation has two parts. Part one is an overview of AD and DLB. The second part summarizes author's research work. The main research aims corroborated in this thesis are three-fold: First, to...
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Štítná žláza prasat
ŠTĚCHOVÁ, Petra
Thyroid gland is very important endocrine organ that interferes with a number of processes in the body. Sufficient iodine saturation of animals is essential for optimal production of thyroid hormones. The aim of the bachelor's thesis was to determine iodine supply of slaughter pigs and morphometric parameters of thyroid glands in selected individuals. The study included 27 castrated hybrids of Czech landrace and Pietrain. Iodine content in urine was determined using the Sandell - Kolthoff spectrophotometric method. Thyroid parameters were determined microscopically: length, width, outer and inner area of the follicles and thyrocyte height. The representation of individual size categories of follicles was also determined. The average content of urinary iodine in the observed group was 350.8+-184.3 ug/l, the maximum was 608 ug/l and the minimum was 76 ug/l. Optimally saturated with iodine was 44.44 % of individuals, 11.11 % had a deficiency and 44.44 % an excess of iodine. The average length of the thyroid follicle was 149.3+-64.8 um, width 105.9+-50.6 um, outer area 16810.6+-18030.0 um2, inner area 14318.4+-16985.2 um2 and thyrocyte height 6.2+-2.6 um. In the thyroid glands was 9.4 % small, 62.8 % medium and 27.8 % large follicles. In the selected group of individuals, the impact of different iodine saturation of animals on all monitored thyroid parameters was demonstrated.
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Postnatální změny ledvin králíka
ROČEŇOVÁ, Adéla
The kidneys are the main excretory organ that completes morphological and functional development in a species-different postnatal period of an individual's life. This bachelor thesis dealt with morphometric and histological changes in the kidneys of rabbits. The study included 42 New Zealand white rabbits at the age of 0, 5, 10, 14, 19, 27, 32 days. During this period, the length, width and weight of the kidneys and histological changes were determined, and at the age of 14, 19, 27, 32 days also the length, width, area and number of renal corpusles. In newborn rabbits, the average kidneys length was 9.75+-0.66 mm and the width was 5.88+-0.78 mm. Over 32 days, length increased by 117.44% (21.2+-1.71 mm) and width by 144.90 % (14.4+-1.61 mm). The most significant increase in kidneys length and width occurred in the first 19 days of life (length 110.26 %, width 144.56 %). The average kidneys weight of the newborn rabbit was 0.25+-0.03 g and 2.40+-0.83 g on day 32. A nephrogenic zone and nephrons type I and II were observed in the kidneys up to 10 days of age. By day 19, nephrons type III were present. The onset of marrow differentiation into inner and outer zones was noted at 10 days and at 14 days the zones were fully distinguishable. In 14-day-old individuals, the average length of renal corpusles was 55.10+-6.32 um, width 67.51+-8.91 um and area 3125.25+-778.99 um2. In 32 days, the length reached 76.90+-6.26 um, the width 93.96+-6.10 um and the area 5963.06+-851.05 um2. High correlation coefficients ranging from 0.886 to 0.972 were found between body weight and renal morphometric parameters. High correlation coefficients were found also between the weight of the kidneys and the morphometric parameters of the renal corpuscles. A negative coefficient was found between the area and the number of renal corpuscles (rxy = -0.997).
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Morphometric disparity in European lineages of gobies (Gobiidae)
Bílá, Kristina Beatrix ; Vukićová, Jasna (advisor) ; Frýdlová, Petra (referee)
Gobies (Actinopterygii: Gobiiformes), are a remarkable group of fishes, not only because they are the most species-rich order of bony fishes (Teleostei), but also due to their extraordinary ability to colonize different types of environments. As a consequence, they evolved a variety of morphological shapes. The aim of this work was to reveal whether the European evolutionary lineages of gobies evolved ecomorphs through repeated colonisation of the same types of microhabitats, i.e. whether morphology (body shape) is influenced by niche, and what role convergent evolution plays in adaptation to different types of environments. For this purpose i made quantification and comparison of body shape using the methods of geometric morphometry. The results of this work showed that convergent evolution has occurred in four out of the six ecological groups of studied gobies, i.e., that their shape is influenced by the environment they inhabit and their way of life, regardless of lineage affiliation and phylogenetic relationships. Convergent evolution has been revealed in hyperbenthic, nektonic, marine epibenthic and freshwater epibenthic species. In contrast, convergent evolution has not been proven in euryhaline epibenthic species, nor in cryptobenthic species of gobies. Key words: geometric morphometry,...
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Experimental model of the ventral spinal cord lesion in rats.
Štěpánková, Kateřina ; Machová Urdzíková, Lucia (advisor) ; Martončíková, Marcela (referee)
A spinal cord injury (SCI) is a damage to the spinal cord that causes permanent or temporary changes in motor and sensory functions. In humans, the traumatic impact to spinal cord is mostly directed from the ventral part of the spinal column, even though SCI models are principally directed from the dorsal part of the spinal column due to easier surgery. The aim of the work described in this thesis was to develop clinically more relevant, easily reproducible and relatively inexpensive model of the ventral spinal cord lesion in rats which replicates SCI in humans as closely as possible by its pathology, completeness, level and regeneration. For the surgery we used a modification of a balloon- compression technique. The balloon of the 2F embolectomy Fogarty's catheter was placed to the anterior epidural space via laminectomy at the level of T10 and when the final position of the catheter was achieved at the spinal level T8 in front of the anterior median fissure, a balloon was rapidly inflated with 10 μl or 15 μl of water for 5 minutes. Other two groups - laminectomy only and ventral placement of the catheter without inflation were used as controls. The motor functions were evaluated by the BBB test and ladder walking test. In BBB test we observed significantly impaired motor functions in the 15 μl...
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Craniometric study of Central European populations of the white-toothed shrews Crocidura suaveolens and C. leucodon (Mammalia: Soricomorpha)
Milerová, Petra ; Vohralík, Vladimír (advisor) ; Anděra, Miloš (referee)
Interspecific and intraspecific size variability of the skull, mandibles and teeth of the lesser white- toothed shrew Crocidura suaveolens and the bicolored white-toothed shrew C. leucodon was studied on material of 350 skulls and 465 mandibles of white-toothed shrews obtained by analysis of the barn owl pellets originating from four regions in the Czech Republic and one region in the southern Slovakia. C. suaveolens was present in all regions under study, while sympatric C. leucodon occurred in three regions only. In total, 21cranial and dental measurements and 4 mandibular measurements were investigated. It was found that the most reliable measurement enabling discrimination between skulls of both species in the whole studied material is the lenght of the lower dental row. If skulls of both species were evaluated separately for each area of their sympatry it was possible to use also the distance between second molars M2 and the width of premolar P4 . Skulls of these two species can also be distinguished by bivariate graphs using the length and width of premolar P4 and the palatal length. We studied relationships between cranial measurements of both species and geoclimatic factors such as longitude, latitude, mean annual temperature and mean annual percipitation. It was found that variability of...
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Hydrological regime of selected lakes in the High Tatra Mts.
Sankotová, Tereza ; Šobr, Miroslav (advisor) ; Janský, Bohumír (referee)
This diploma thesis deals with morphometric analysis and hydrological regime of Skalnaté pleso lake and Hincové Oká lakes, especially the lake level fluctuation, during the period 2011 - 2014. One of the tasks was the bathymetric mapping of observed locations. For Hincové Oká lakes was it the first mapping of bathymetry at all. The hydrological regime and lake level fluctuation regime of observed locations shows interesting differences from the other Tatra lakes whose hydrological regime is controlled by precipitation amounts. The annual culmination of Hincové Oká lakes (the largest lake) is shifted into the spring caused by melting snowpack. When there is no rainfall for a couple of days Skalnaté pleso lake often dries up, even during summer months, when levels of most Tatra lakes reach their maximum values. However summer drying is shortterm. Lake level fluctuation regime is very dynamic. After a strong rainfall event its level rises very steeply, up to 0,14 m per hour. Powered by TCPDF (www.tcpdf.org)
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